Anti-CD3 Anticorpo [KT3] (PE) (A280836)

Anti-CD3 Antibody [KT3] (PE)

Rat monoclonal [KT3] antibody to CD3 (PE).

This antibody recognises the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.

Flow Cytometry

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Mouse

CBAT6 thymocytes.

Rat

Monoclonal

KT3

IgG2a

PE

Excitation: 565nm, Emission: 578nm

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD3 antibody, clone KT3 recognizes the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.

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