This antibody recognises the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.

Applicazioni

Flow Cytometry

Diluizioni consigliate

Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Reattività

Mouse

Immunogeno

CBAT6 thymocytes.

Specie ospite

Rat

Clonalità

Monoclonal

Clona ID

KT3

Isotipo

IgG2a

Coniugare

FITC

Excitation: 490nm, Emission: 525nm

Concentrazione

100 µg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conservazione

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Note generali

Rat anti Mouse CD3 antibody, clone KT3 recognizes the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.

Sinonimi

4930549J05Rik, A430104F18Rik, AW552088, Cd247, CD247 antigen, CD247 antigen, zeta subunit, CD247 molecule, CD3 antigen, delta subunit, CD3 delta, CD3 epsilon, CD3 eta, CD3 gamma, CD3 molecule delta polypeptide, CD3 molecule, epsilon polypeptide, CD3 molecule, gamma polypeptide, CD3 zeta, CD3-DELTA, CD3d, CD3D antigen delta polypeptide, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule delta, CD3d molecule delta CD3 TCR complex, CD3d molecule, delta (CD3-TCR complex), CD3D_HUMAN, CD3E, CD3e antigen, CD3E antigen epsilon polypeptide, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule epsilon, CD3e molecule epsilon CD3 TCR complex, CD3e molecule, epsilon (CD3-TCR complex), CD3epsilon, CD3G, CD3g antigen, CD3G antigen gamma polypeptide, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule gamma, CD3g molecule gamma CD3 TCR complex, CD3g molecule, gamma (CD3-TCR complex), CD3H, CD3Q, CD3Z, CD3zeta, Ctg3, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD19, Leu-4, MGC138597, OKT3, delta chain, OTTHUMP00000032544, T cell receptor, T cell receptor T3 delta chain, T cell receptor T3 gamma chain, T cell receptor T3 zeta chain, T cell receptor zeta chain, T cell surface glycoprotein CD3, T cell surface glycoprotein CD3 delta chain, T cell surface glycoprotein CD3 epsilon chain, T cell surface glycoprotein CD3 gamma chain, T cell surface glycoprotein CD3 zeta chain, T-cell antigen receptor complex, delta subunit of T3, T-cell antigen receptor complex, epsilon subunit of T3, T-cell antigen receptor complex, gamma subunit of T3, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 delta chain, T-cell receptor T3 gamma chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 gamma chain, T3, T3d, T3e, T3g, T3z, TCRE, TCRk, Tcrz, TCRzeta

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