This antibody recognises the rhesus monkey RhT3 antigen, the homolog of human CD3. Mouse anti Monkey CD3 antibody, clone FN-18 has been reported to recognize a polymorphic epitope of the RhT3 antigen, which is expressed by mature T lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:10, Use 50µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Monkey, Macaque, Cynomolgus monkey, African green monkey
Cross Reactivity
This antibody does not cross react with Chimpanzee, Gorilla, or Marmoset
Immunogen
Rhesus monkey peripheral blood leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
FN-18
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Ion exchange chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.5 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% Bovine Serum Albumin is recommended.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 125mM Trehalose.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Monkey CD3 antibody, clone FN-18 recognizes the rhesus monkey RhT3 antigen, the homolog of human CD3. Mouse anti Monkey CD3 antibody, clone FN-18 has been reported to recognize a polymorphic epitope of the RhT3 antigen, which is expressed by mature T lymphocytes.