Rabbit polyclonal antibody to CD28 (phospho Tyr218).
Specificità
This antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
Applicazioni
WB, ELISA
Diluizioni consigliate
WB: 1:500-1:1000, ELISA: 1:40000
Reattività
Human, Mouse
Immunogeno
Synthetic peptide derived from human CD28 around the phosphorylation site of Tyr218 (amino acids 171-220).
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Peso molecolare
25kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44
Figure 1: Western Blot - Anti-CD28 (phospho Tyr218) Antibody (A94934)
Western blot analysis of lysates from HepG2 cells treated with nocodazole 1µg/ml 16h using Anti-CD28 (phospho Tyr218) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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