This antibody recognises mouse CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Applicazioni
Flow Cytometry, Functional Assays
Diluizioni consigliate
Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reattività
Mouse
Immunogeno
Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
E18
Isotipo
IgG2b
Coniugare
FITC
Excitation: 490nm, Emission: 525nm
Purificazione
Protein A affinity chromatography of tissue culture supernatant.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline.
Conservazione
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
Note generali
Mouse anti Mouse CD28 antibody, clone E18 recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Sinonimi
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44