Recombinant rabbit monoclonal [DM64] antibody to CD28.
Applicazioni
ELISA, Flow Cytometry
Diluizioni consigliate
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reattività
Human
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
DM64
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Affinity chromatography.
Concentrazione
Reconstitution dependent.
Forma del prodotto
Lyophilized
Ricostituzione
Reconstitute with distilled sterile water.
Formulazione
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Note generali
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Sinonimi
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44
ELISA plates were pre-coated with Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) at 2 µg/ml (100 µl/well) which can bind Anti-CD28 Antibody [DM64] - BSA and Azide free (A318633) in a linear range of 1-100 ng/ml.
Expi 293 cell line was transfected with irrelevant protein (A) and human CD28 (B) were surface stained with Anti-CD28 Antibody [DM64] - BSA and Azide free (A318633) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Flow cytometry data of serially titrated Anti-CD28 Antibody [DM64] - BSA and Azide free (A318633) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit Anti-CD28 Monoclonal Antibody clones by titration of different concentrations onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Prodotti alternativi a Anti-CD28 Anticorpo [DM64] (A318633)