This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Applicazioni

Flow Cytometry, IF

Diluizioni consigliate

Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100

Reattività

Human

Immunogeno

Recombinant human CD28 protein.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

CB28

Isotipo

IgG1

Catene leggere

kappa

Coniugare

Excitation: 565nm, Emission: 578nm

Purificazione

Protein A/G chromatography.

Concentrazione

100 µg/ml

Peso molecolare

44 kDa (monomer) / 88 kDa (dimer)

Forma del prodotto

Liquid

Formulazione

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conservazione

Store at 4-8°C. Antibody is stable for 24 months.

Sinonimi

CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44

Controlli isotipo

Mouse IgG1 [MOPC-21] (PE) (A86199)

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (1)

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Figure 1: SDS-PAGE - Anti-CD28 Antibody [CB28] (PE) (A251160)

SDS-PAGE analysis of Anti-CD28 Antibody [CB28] (PE) under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.