Recombinant rabbit monoclonal [DM53] antibody to CD276.
Applicazioni
ELISA, Flow Cytometry
Diluizioni consigliate
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reattività
Human
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
DM53
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Affinity chromatography.
Concentrazione
Reconstitution dependent.
Forma del prodotto
Lyophilized
Ricostituzione
Reconstitute with distilled sterile water.
Formulazione
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Note generali
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Flow cytometry data of serially titrated Anti-CD276 Antibody [DM53] - Azide free (A318643) on on Expi 293 cell line transfected with human B7-H3. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Expi 293 cell line transfected with irrelevant protein (A) and human B7-H3 (B) were surface stained with Anti-CD276 Antibody [DM53] - Azide free (A318643) at 1 µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Prodotti alternativi a Anti-CD276 Anticorpo [DM53] (A318643)