Anti-CD276 Antibody [DM53] (Biotin) - BSA and Azide free
Descrizione
Recombinant rabbit monoclonal [DM53] antibody to CD276 (Biotin).
Applicazioni
ELISA, Flow Cytometry
Diluizioni consigliate
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reattività
Human
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
DM53
Isotipo
IgG
Coniugare
Biotin
Purificazione
Affinity chromatography.
Concentrazione
Reconstitution dependent.
Forma del prodotto
Lyophilized
Ricostituzione
Reconstitute with distilled sterile water.
Formulazione
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Note generali
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
1e5 of Expi 293 cell line were stained with 100µl of 1:1,000 diluted Anti-CD276 Antibody [DM53] (Biotin) - Azide free (A318642) (blue histogram) or isotype control (red histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.
![Flow Cytometry - Anti-CD276 Antibody [DM53] (Biotin) - Azide free (A318642) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318642_1.jpg?profile=product_top)
![Flow Cytometry - Anti-CD276 Antibody [DM53] (Biotin) - Azide free (A318642) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318642_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD276 Antibody [DM53] (Biotin) - Azide free (A318642) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318642_1.jpg?profile=product_image)