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Anti-CD27 Anticorpo (A13763)

Nome
Anti-CD27 Antibody
Descrizione
Rabbit polyclonal antibody to CD27.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1).
Sequenza
ATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIR
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
55 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CD 27, CD27 antigen, CD27 molecule, CD27L receptor, CD27_HUMAN, LPFS2, MGC20393, OTTHUMP00000238557, S152, T cell activation antigen CD27, T cell antivation antigen S152, T-cell activation antigen CD27, T14, TNFRSF 7, TNFRSF7, TNFSF7, Tp 55, Tp55, Tumor necrosis factor receptor superfamily member 7
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CD27 Antibody (A13763) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CD27 Antibody (A13763)
Western blot analysis of extracts of Raji cells, using Anti-CD27 Antibody (A13763) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-CD27 Antibody (A13763) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-CD27 Antibody (A13763)
Western blot analysis of extracts of various cell lines, using Anti-CD27 Antibody (A13763) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-CD27 Antibody (A13763) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Western Blot - Anti-CD27 Antibody (A13763)
Western blot analysis of extracts of Rat lung, using Anti-CD27 Antibody (A13763) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-CD27 Antibody (A13763) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-CD27 Antibody (A13763)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD27 Antibody (A13763) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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