Mouse monoclonal [OX-104] antibody to CD200/OX2 (PE-Cyanine 7).
Specificità
This antibody recognizes an extracellular epitope of CD200, a type-1 glycoprotein of the immunoglobulin superfamily, which is expressed in neurons, B and T cell subsets, keratinocytes, follicular dendritic cells, and ovarian cells.
Applicazioni
Flow Cytometry
Diluizioni consigliate
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reattività
Human
Immunogeno
Human CD200.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
OX-104
Isotipo
IgG1
Catene leggere
kappa
Coniugare
PE-Cyanine 7
Excitation: 565nm, Emission: 778nm
Purificazione
This antibody is conjugated with tandem dye PE-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Conservazione
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD200 Antibody [OX-104] (PE-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).
Flow cytometric analysis of human lymphocytes stained using Anti-CD200 Antibody [OX-104] (PE-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood) and Anti-CD19 Antibody [LT19] (APC) (10µl reagent per 100µl of peripheral whole blood).
Separation of human CD200 positive B-cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD200 Antibody [OX-104] (PE-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).