Mouse monoclonal [GHI/61] antibody to CD163 (APC).
Specificity
This antibody recognizes CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Hairy cell leukemia cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
GHI/61
Isotype
IgG1
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
130 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
Hemoglobin scavenger receptor, M130, Scavenger receptor cysteine-rich type 1 protein M130
Surface staining of human peripheral blood using Anti-CD163 Antibody (A86215). CD163 was detected on CD14+ monocytes, whereas CD14- lymphocytes were used as a negative control.
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