Mouse monoclonal [Ber-ACT35] antibody to CD134/OX40L Receptor (APC).
Specificità
This antibody, also known as clone ACT35, recognizes an extracellular epitope of CD134, an approximately 50 kDa type I transmembrane glycoprotein expressed on activated T cells.
Applicazioni
Flow Cytometry
Diluizioni consigliate
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reattività
Human, Non-Human Primates
Immunogeno
HTLV 1 transformed HUT-102 cells.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
Ber-ACT35
Isotipo
IgG1
Catene leggere
kappa
Coniugare
APC
Excitation: 645nm, Emission: 660nm
Purificazione
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
MW previsto
50 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Conservazione
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human PHA stimulated peripheral blood mononuclear cells stained with Anti-CD134 Antibody [Ber-ACT35] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
Flow cytometric analysis of human PHA stimulated peripheral blood mononuclear cells stained using Anti-CD134 Antibody [Ber-ACT35] (APC) (10µl reagent per milion cells in 100µl of cell suspension) and Anti-CD25 Antibody [MEM-181] (PE) (20µl reagent per 100µl of peripheral whole blood).
Separation of human CD134 positive CD25 positive cells (red-filled) from CD134 negative CD25 negative cells (black-dashed) in flow cytometry analysis of human PHA stimulated peripheral blood mononuclear cells using Anti-CD134 Antibody [Ber-ACT35] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
![Flow Cytometry - Anti-CD134 Antibody [Ber-ACT35] (APC) (A121883) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121883_1.jpg?profile=product_top)
![Flow Cytometry - Anti-CD134 Antibody [Ber-ACT35] (APC) (A121882) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121883_2.jpg?profile=product_top)
![Flow Cytometry - Anti-CD134 Antibody [Ber-ACT35] (APC) (A121883) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121883_3.jpg?profile=product_top)
![Flow Cytometry - Anti-CD134 Antibody [Ber-ACT35] (APC) (A121883) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121883_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD134 Antibody [Ber-ACT35] (APC) (A121882) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121883_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD134 Antibody [Ber-ACT35] (APC) (A121883) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121883_3.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD134 Antibody [Ber-ACT35] (APC) (A121883) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121883_1.jpg?profile=product_image)
![Flow Cytometry - Anti-CD134 Antibody [Ber-ACT35] (APC) (A121882) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121883_2.jpg?profile=product_image)
![Flow Cytometry - Anti-CD134 Antibody [Ber-ACT35] (APC) (A121883) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121883_3.jpg?profile=product_image)