Figure 1: Western Blot - Anti-Cathepsin D Antibody (A89998)
Western blot analysis of SK-BR-3, using Anti-Cathepsin D Antibody (A89998) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Figure 2: Western Blot - Anti-Cathepsin D Antibody (A89998)
Western blot analysis of Rat lung, using Anti-Cathepsin D Antibody (A89998) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Figure 3: Immunohistochemistry - Anti-Cathepsin D Antibody (A89998)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Cathepsin D Antibody (A89998) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 4: Immunohistochemistry - Anti-Cathepsin D Antibody (A89998)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Cathepsin D Antibody (A89998) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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