Anti-Caspase-3 Anticorpo (A83337)

Name: Anti-Caspase-3 Antibody
Brand: Antibodies.com
SKU: A83337
Price: 480.0 USD
Availability: InStock

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$480

Anticorpo policlonale di capra contro Caspase-3 per ELISA, WB e IF/ICC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-Caspase-3 Antibody

Descrizione

Goat polyclonal antibody to Caspase-3.

Applicazioni

ELISA, WB, IF/ICC

Diluizioni consigliate

ELISA: 1:32,000, WB: 0.5-1µg/ml, IF: 10µg/ml

Reattività

Human, Mouse, Rat

Immunogeno

Synthetic peptide corresponding to the internal region of human Caspase-3.

Sequenza

C-RDVSKEDHSKRS

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

32 kDa

MW previsto

31.6kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

A830040C14Rik, Apopain, CASP-3, CASP3, Casp3a, CASP3_HUMAN, Caspase 3, Caspase 3, apoptosis-related cysteine peptidase, Caspase 3, apoptosis-related cysteine protease, Caspase 3, apoptosis-related cysteine protease a, Caspase-3 subunit p12, CC3, CPP-32, CPP32, CPP32B, Cysteine protease CPP32, EC 3.4.22.56, LICE, mldy, OTTHUMP00000165052, OTTHUMP00000165053, OTTHUMP00000165054, PARP cleavage protease, Procaspase3, Protein Yama, SCA 1, SCA-1, SREBP cleavage activity 1, Yama

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-Caspase-3 Antibody (A83337)

Caspase-3 expression in MOLT4 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Caspase-3 Antibody (A83337) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-Caspase-3 Antibody (A83337)

Caspase-3 expression in Mouse Liver lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Caspase-3 Antibody (A83337) at 0.5µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-Caspase-3 Antibody (A83337)

Caspase-3 expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Caspase-3 Antibody (A83337) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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