Western Blot - Anti-Cardiac Troponin I Antibody (A89157)
Western blot analysis of Mouse heart, using Anti-Cardiac Troponin I Antibody (A89157) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.
Western Blot - Anti-Cardiac Troponin I Antibody (A89157)
Western blot analysis of Rat lung, using Anti-Cardiac Troponin I Antibody (A89157) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence - Anti-Cardiac Troponin I Antibody (A89157)
Immunofluorescence analysis of mouse heart cells using Anti-Cardiac Troponin I Antibody (A89157) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-Cardiac Troponin I Antibody (A89157)
Immunoprecipitation analysis of 600µg extracts of mouse heart cells using 3µg of Anti-Cardiac Troponin I Antibody (A89157). This Western blot was performed on the immunoprecipitate using Anti-Cardiac Troponin I Antibody (A89157) at a dilution of 1:1000.
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