Anti-CaMKII Anticorpo [22B1] (A305070)

Name: Anti-CaMKII Antibody [22B1]
Brand: Antibodies.com
SKU: A305070
Price: 465.0 USD
Availability: InStock

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$465

Anticorpo monoclonale [22B1] di topo contro CaMKII per WB, IHC, ICC/IF, IP e ELISA.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-CaMKII Antibody [22B1]

Descrizione

Mouse monoclonal [22B1] antibody to CaMKII.

Specificità

This antibody detects phosphorylated CaMKII from rat tissues. This antibody is specific for a and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta).

Applicazioni

WB, IHC, ICC/IF, IP, ELISA

Diluizioni consigliate

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

Synthetic peptide to Rat CaMKII.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

22B1

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Calcium/calmodulin dependent protein kinase II alpha, Calcium/calmodulin dependent protein kinase II beta, Calcium/calmodulin dependent protein kinase II delta, Calcium/calmodulin dependent protein kinase II gamma, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II, CaM kinase II alpha, CaM kinase II beta, CaM kinase II delta, CaM kinase II gamma, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2, Camk2a, CAMK2B, CAMK2D, CAMK2G, CAMKA, KCC2A_HUMAN

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-CaMKII Antibody [22B1] (A305070)

Western blot analysis of mouse Ventricle lysates showing detection of CaMKII protein using Anti-CaMKII Antibody [22B1] (A305070) at 1:1,000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.

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Figure 2: Immunohistochemistry - Anti-CaMKII Antibody [22B1] (A305070)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-CaMKII Antibody [22B1] (A305070) at 1:5,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Magnification: 40x.

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Figure 3: Immunohistochemistry - Anti-CaMKII Antibody [22B1] (A305070)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CaMKII Antibody [22B1] (A305070) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.

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Figure 4: Immunofluorescence - Anti-CaMKII Antibody [22B1] (A305070)

Immunocytochemistry/Immunofluorescence analysis of rat dissociated hippocampal neurons, fixed in cold 4% paraformaldehyde / 0.2% glutaraldehyde in 0.1M sodium phosphate buffer, using Anti-CaMKII Antibody [22B1] (A305070), at 1:1,000 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at room temperature. Magnification: 10X.