Figure 1: Western Blot - Anti-Calpain 5 Antibody (A15689)
Western blot analysis of extracts of various cell lines, using Anti-Calpain 5 Antibody (A15689) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
Immunofluorescence analysis of NIH-3T3 cells using Anti-Calpain 5 Antibody (A15689) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Prodotti alternativi a Anti-Calpain 5 Anticorpo (A15689)