Figure 1: Western Blot - Anti-C3a R Antibody (A9843)
Western blot analysis of extracts of various cell lines, using Anti-C3a R Antibody (A9843) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.
