Anti-Brd4 (phospho Thr204) Anticorpo [ARC50960] (A305446)

Name: Anti-Brd4 (phospho Thr204) Antibody [ARC50960]
Brand: Antibodies.com
SKU: A305446
Price: 540.0 USD
Availability: InStock

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$540

Anticorpo monoclonale [ARC50960] di coniglio contro Brd4 (phospho Thr204) per WB.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Brd4 (phospho Thr204) Antibody [ARC50960]

Descrizione

Rabbit monoclonal [ARC50960] antibody to Brd4 (phospho Thr204).

Applicazioni

Diluizioni consigliate

WB: 1:500-1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around T204 of human BRD4 (NP_490597.1).

Sequenza

ASTPP

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC50960

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

200 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

BRD4-NUT FUSION, BRD4-NUT fusion oncoprotein, BRD4_HUMAN, Bromodomain containing 4, bromodomain containing protein 4, Bromodomain-containing protein 4, CAP, chromosome associated protein, HUNK1, HUNKI, MCAP, Mitotic chromosome-associated protein, Protein HUNK1

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (2)

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Figure 1: Western Blot - Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446)

Western blot analysis of extracts of various cell lines, using Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446) at 1:1,000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) for 30 minutes with NaCl (400mM) after serum-starvation 16-20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.