Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Name: Anti-BRCA1 (phospho Ser1423) Antibody
Brand: Antibodies.com
SKU: A10901
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to BRCA1 (phospho Ser1423) for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-BRCA1 (phospho Ser1423) Antibody

Description

Rabbit polyclonal antibody to BRCA1 (phospho Ser1423).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S1423 of human BRCA1 (NP_009225.1).

Sequence

HGSQP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

270 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, RNF53

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Western blot analysis of extracts of HT-29 cells, using Anti-BRCA1 (phospho Ser1423) Antibody (A10901) at 1:2,000 dilution. HT-29 cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-BRCA1 (phospho Ser1423) Antibody (A10901) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-BRCA1 (phospho Ser1423) Antibody (A10901) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-BRCA1 (phospho Ser1423) Antibody (A10901) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.