Anti-Bif-1 Anticorpo (A83782)

Name: Anti-Bif-1 Antibody
Brand: Antibodies.com
SKU: A83782
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Novus (NB100-820). Scopri di più con meno.

Clicca sull'immagine per la visualizzazione estesa

$480

Anticorpo policlonale di capra contro Bif-1 per ELISA, WB, IHC e Flow Cytometry.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

Garanzia di corrispondenza del prezzo

Descrizione Prodotto

Nome

Anti-Bif-1 Antibody

Descrizione

Goat polyclonal antibody to Bif-1.

Applicazioni

ELISA, WB, IHC, Flow Cytometry

Diluizioni consigliate

ELISA: 1:32,000, WB: 0.1-0.3µg/ml, IHC: 2.5µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human, Mouse, Rat

Immunogeno

Synthetic peptide corresponding to the C terminal region of human Bif-1.

Sequenza

C-QKGKVPITYLELLN

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

40 kDa

MW previsto

40.8kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Bax interacting factor 1, Bax-interacting factor 1, Bif, Bif 1, CGI 61, dJ612B15.2, Endophilin B1, Endophilin-B1, PPP1R70, Protein phosphatase 1 regulatory subunit 70, SH3 containing protein SH3GLB1, SH3 domain GRB2 like endophilin B1, SH3 domain-containing GRB2-like protein B1, Sh3glb1, SH3p13, SHLB1_HUMAN

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

Clicca sull'immagine per la visualizzazione estesa

Figure 1: Western Blot - Anti-Bif-1 Antibody (A83782)

Bif-1 expression in HEK293 (A) and A431 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Bif-1 Antibody (A83782) at 0.1µg/ml and detected by chemiluminescence.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Western Blot - Anti-Bif-1 Antibody (A83782)

Bif-1 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Bif-1 Antibody (A83782) at 0.1µg/ml and detected by chemiluminescence.

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Flow Cytometry - Anti-Bif-1 Antibody (A83782)

Bif-1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Bif-1 Antibody (A83782) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

Clicca sull'immagine per la visualizzazione estesa

Figure 4: Immunohistochemistry - Anti-Bif-1 Antibody (A83782)

Bif-1 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Bif-1 Antibody (A83782) at 2.5µg/ml and revealed with alkaline phosphatase (AP).