Mouse monoclonal [TU-20] antibody to beta III Tubulin.
Specificity
This antibody recognises class III beta-tubulin, restricted to neuronal tissue (Leandro-García 2010; Katsetos 2003). Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin (Jirásek 2002) including neuroblastoma (Prasannan 2000) and ganglioneuroma (Dráberová 1998). Class III beta tubulin is highly expressed in tumors of neuronal origin rather than in non-neuronal tumors (Person 2017).
Applications
WB, IHC-P, IHC-Fr, IF
Dilutions
IHC-P 1: 5 - 20 µg/ml, WB 2: 1 - 10 µg/ml
Reactivity
Human, Mouse, Baboon, Rat, Hamster, Pig, Bovine
Immunogen
Synthetic peptide sequence ESESQGPK corresponding to amino acids 441-448 of human class III beta tubulin coupled to Keyhole Limpet Haemocyanin. This sequence is widely conserved across species.
Sequence
ESESQGPK
Host
Mouse
Clonality
Monoclonal
Clone ID
TU-20
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Tubulin beta 3 antibody, clone TU-20 recognizes class III beta-tubulin, restricted to neuronal tissue (Leandro-García 2010; Katsetos 2003).Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin (Jirásek 2002) including neuroblastoma (Prasannan 2000) and ganglioneuroma (Dráberová 1998). Class III beta tubulin is highly expressed in tumors of neuronal origin rather than in non-neuronal tumors (Person 2017).