Anti-B7H4 Anticorpo (A84379)

Name: Anti-B7H4 Antibody
Brand: Antibodies.com
SKU: A84379
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Novus (NBP1-06096). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro B7H4 per ELISA, WB, IF e Flow Cytometry.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-B7H4 Antibody

Descrizione

Goat polyclonal antibody to B7H4.

Applicazioni

ELISA, WB, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:64,000, WB: 0.1-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region of human B7H4.

Sequenza

C-SKGKGNANLEYK

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

26 kDa and 21 kDa

MW previsto

30.9kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

B7 family member, H4, B7 H4, B7 homolog 4, B7 superfamily member 1, B7 superfamily, member 1, B7-H4, B7h.5, B7S1, B7x, BC032925, Immune costimulatory protein B7-H4, Immune costimulatory protein B7H4, MGC41287, PRO1291, Protein B7S1, RP11 229A19.4, T cell costimulatory molecule B7x, T-cell costimulatory molecule B7x, V set domain-containing T cell activation inhibitor 1, V-set domain-containing T-cell activation inhibitor 1, VCTN1, Vtcn1, VTCN1_HUMAN

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-B7H4 Antibody (A84379)

B7H4 expression in HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B), Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D), and Human pancreas iso 1 (E) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-B7H4 Antibody (A84379) at 1µg/ml (A-D) or 0.1µg/ml (E) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-B7H4 Antibody (A84379)

B7H4 expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-B7H4 Antibody (A84379) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane/cell junction staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-B7H4 Antibody (A84379)

B7H4 expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-B7H4 Antibody (A84379) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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