Anti-Aurora A + B Kinase Anticorpo [3H1] (A85400)

HeLa cell cultures were stained with Anti-Aurora A/B Kinase Antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with Anti-Vimentin Antibody (A85421 | red). Blue is a DNA stain.

Left: Western blot analysis of Anti-Aurora A/B Kinase Antibody in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Anti-Aurora A/B Kinase Antibody. Nocodazole is a microtubule depolymerizing agent which induces cells to halt at the G2/M phase and also induces Aurora A expression. The Anti-Aurora A/B Kinase Antibody binds strongly to a band at about 46 kDa, which is Aurora A and also shows binding to a band at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with Anti-Aurora A/B Kinase Antibody, which binds to both Aurora A and B. We conclude that the 38 kDa band seen in the HeLa extract is Aurora B. This is also consisted with the immunocytochemical staining of both centrosomes and midbodies seen on HeLa cells.

Western blot analysis of different cell lysates and recombinant protein solutions using Anti-Aurora A + B Kinase Antibody [3H1] (A85400), in green. Left: Cells were treated with 100 ng/mL of nicodazol for 6 hours. The lanes contain samples of: [1] Protein standards, in red, [2] HeLa cells, [3] A72 cells, [4] NBL6 cells, and [5] KR158 cells. Right: human recombinant protein Aurora (A, B, C as indicated) solutions. Bands at 46kDa and 38kDa correspond to Aurora A and Aurora B proteins respectively.