Anti-ATR (phospho Ser428) Anticorpo (A91791)

Name: Anti-ATR (phospho Ser428) Antibody
Brand: Antibodies.com
SKU: A91791
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro ATR (phospho Ser428) per WB, IHC e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-ATR (phospho Ser428) Antibody

Descrizione

Rabbit polyclonal antibody to ATR (phospho Ser428).

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).

Sequenza

GISPK

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

300 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Ataxia telangiectasia and Rad3 related, Ataxia telangiectasia and Rad3-related protein, ATR_HUMAN, FCTCS, FRAP Related Protein 1, FRAP-related protein 1, FRP1, MEC1, MEC1 mitosis entry checkpoint 1 homolog, Protein kinase ATR, RAC3, Rad3 related protein, SCKL, SCKL1, Serine/threonine protein kinase ATR, Serine/threonine-protein kinase ATR

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

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Figure 1: Western Blot - Anti-ATR (phospho Ser428) Antibody (A91791)

Western blot analysis of 293T, using Anti-ATR (phospho Ser428) Antibody (A91791) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-ATR (phospho Ser428) Antibody (A91791)

Western blot analysis of NIH/3T3, using Anti-ATR (phospho Ser428) Antibody (A91791) at 1:400 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-ATR (phospho Ser428) Antibody (A91791)

Immunohistochemistry analysis of paraffin-embedded human testis using Anti-ATR (phospho Ser428) Antibody (A91791) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ATR (phospho Ser428) Antibody (A91791)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-ATR (phospho Ser428) Antibody (A91791) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.