ATP1A1 Cell Based Kit Elisa (A102991)

Nome
ATP1A1 Cell Based ELISA Kit
Tipo di kit
Cell-based (qualitative)
Principio di kit
This ATP1A1 Cell Based ELISA Kit allows for the detection of ATP1A1 and the effects that certain stimulation conditions have on ATP1A1 expression in different cell lines. Qualitative determination of ATP1A1 concentration is achieved by an indirect ELISA format. In essence, the ATP1A1 is captured by Anti-ATP1A1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this ATP1A1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Adherent cells and suspension cells.
Campo di rilevamento
> 5000 Cells
Tempo del kit
4 hours 30 minutes
Reattività
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at + 4°C. Stable for 6 months.
Sinonimi
adenosinetriphosphatase, AT1A1_HUMAN, ATP1A4, ATP1AL2, ATP1B, ATP1B1, ATPase Na+/K+ transporting alpha, ATPase Na+/K+ transporting alpha 1 polypeptide, ATPase Na+/K+ transporting alpha 4 polypeptide, ATPase Na+/K+ transporting beta 1 polypeptide, ATPase, Na+/K+ transporting, alpha polypeptide-like 2, ATPase, Na+/K+ transporting, beta 1 polypeptide, Beta 1-subunit of Na(+),K(+)-ATPase, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase alpha-4 subunit, Na+, K+ ATPase alpha subunit, Na+/K+ ATPase 1, Na+/K+ ATPase 4, Na+/K+ ATPase, alpha-D polypeptide, Na, K-ATPase beta-1 polypeptide, Na, K-ATPase, alpha-A catalytic polypeptide, Na,K-ATPase catalytic subunit alpha-A protein, Na,K-ATPase subunit alpha-C, polypeptide-like 2, Sodium Potassium ATPase, Sodium pump 1, sodium pump 4, Sodium pump subunit alpha-1, sodium pump subunit alpha-4, sodium pump subunit beta-1, sodium-potassium ATPase catalytic subunit alpha-1, sodium-potassium ATPase catalytic subunit alpha-4, sodium-potassium ATPase subunit beta 1 (non-catalytic), sodium-potassium ATPase, alpha 4 polypeptide, sodium-potassium-ATPase, alpha 1 polypeptide, Sodium/potassium transporting ATPase alpha 1 chain, Sodium/potassium transporting ATPase subunit beta 1, sodium/potassium-dependent ATPase beta-1 subunit, sodium/potassium-transporting ATPase alpha-4 chain, sodium/potassium-transporting ATPase beta-1 chain, Sodium/potassium-transporting ATPase subunit alpha-1, sodium/potassium-transporting ATPase subunit alpha-4
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

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