Anti-ATF6 Anticorpo (A85123)

Name: Anti-ATF6 Antibody
Brand: Antibodies.com
SKU: A85123
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab174756). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro ATF6 per ELISA, WB, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-ATF6 Antibody

Descrizione

Goat polyclonal antibody to ATF6.

Applicazioni

ELISA, WB, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:128,000, WB: 0.3-2µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region, amino acids 437-449, of human ATF6.

Sequenza

C-NSYRYDHSVSNDK

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

75-80 kDa

MW previsto

74.6kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Activating transcription factor 6, Activating transcription factor 6 alpha, ATF 6, ATF6 alpha, ATF6-alpha, ATF6A, ATF6A_HUMAN, cAMP dependent transcription factor ATF 6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Cyclic AMP dependent transcription factor ATF 6 alpha, DKFZp686P2194, ESTM49, FLJ21663, Processed cyclic AMP dependent transcription factor ATF 6 alpha, Processed cyclic AMP-dependent transcription factor ATF-6 alpha

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-ATF6 Antibody (A85123)

ATF6 expression in HeLa (A), MCF7 (B), HeLa nuclear (C), and Jurkat nuclear (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ATF6 Antibody (A85123) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-ATF6 Antibody (A85123)

ATF6 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ATF6 Antibody (A85123) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-ATF6 Antibody (A85123)

ATF6 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ATF6 Antibody (A85123) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-ATF6 Antibody (A85123)

ATF6 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ATF6 Antibody (A85123) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.