Synthetic peptide corresponding to the internal region of human Aryl Hydrocarbon Receptor.
Sequenza
C-PENQKHGLNPQSA
Specie ospite
Goat
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentrazione
500 µg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Sinonimi
Ah receptor, AhR, AHR_HUMAN, Aromatic hydrocarbon receptor, Aryl hydrocarbon receptor precursor, bHLHe76, Class E basic helix loop helix protein 76, Class E basic helix-loop-helix protein 76, HGNC:348
Aryl Hydrocarbon Receptor expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Aryl Hydrocarbon Receptor Antibody (A84652) at 2µg/ml and revealed with horseradish peroxidase (HRP).
Aryl Hydrocarbon Receptor expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Aryl Hydrocarbon Receptor Antibody (A84652) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Aryl Hydrocarbon Receptor expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Aryl Hydrocarbon Receptor Antibody (A84652) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Aryl Hydrocarbon Receptor expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Aryl Hydrocarbon Receptor Antibody (A84652) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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