Figure 1: Western Blot - Anti-Aquaporin 5 Antibody [ARC59536] (A308750)
Western blot analysis of various lysates, using Anti-Aquaporin 5 Antibody [ARC59536] (A308750) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Figure 2: Western Blot - Anti-Aquaporin 5 Antibody [ARC59536] (A308750)
Western blot analysis of various lysates, using Anti-Aquaporin 5 Antibody [ARC59536] (A308750) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Aquaporin 5 Antibody [ARC59536] (A308750) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Aquaporin 5 Antibody [ARC59536] (A308750) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Aquaporin 5 Antibody [ARC59536] (A308750) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.