Figure 1: Western Blot - Anti-Aquaporin 4 Antibody [ARC54345] (A80530)
Western blot analysis of extracts of Rat brain, using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of mouse brain using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of rat brain using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).