Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
AQP-1, AQP-10, AQP1, AQP10, Aquaglyceroporin-10, Aquaporin-1, Aquaporin-10, Aquaporin-CHIP, CHIP28, Small intestine aquaporin, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule
Western Blot - Anti-Aquaporin 1 Antibody [ARC0925] (A306513)
Western blot analysis of extracts of Mouse liver, using Anti-Aquaporin 1 Antibody [ARC0925] (A306513) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Aquaporin 1 Antibody [ARC0925] (A306513)
Western blot analysis of extracts of various cell lines, using Anti-Aquaporin 1 Antibody [ARC0925] (A306513) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Aquaporin 1 Antibody [ARC0925] (A306513) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Aquaporin 1 Antibody [ARC0925] (A306513) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Aquaporin 1 Antibody [ARC0925] (A306513) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.