Mouse monoclonal [APOB/4332] antibody to Apolipoprotein B.
Specificità
Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated byAPOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. ApoB-100 is a protein primarily synthesized in the liver as a structural component of very low density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene.
Applicazioni
IHC-P
Diluizioni consigliate
IHC-P: 1-2 µg/ml
Reattività
Human
Immunogeno
Recombinant fragment, around amino acids 592-689, of human Apolipoprotein B. The exact sequence is proprietary.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
APOB/4332
Isotipo
IgG1
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
200 µg/ml
Peso molecolare
512 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Figure 2: SDS-PAGE - Anti-Apolipoprotein B Antibody [APOB/4332] (A277649)
SDS-PAGE analysis of Anti-Apolipoprotein B Antibody [APOB/4332] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Figure 3: Anti-Apolipoprotein B Antibody [APOB/4332] (A277649)
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-Apolipoprotein B Antibody [APOB/4332]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Prodotti alternativi a Anti-Apolipoprotein B Anticorpo [APOB/4332] (A277649)