Figure 1: Western Blot - Anti-Apc5 Antibody (A15547)
Western blot analysis of extracts of various cell lines, using Anti-Apc5 Antibody (A15547) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Figure 2: Western Blot - Anti-Apc5 Antibody (A15547)
Western blot analysis of extracts from normal (control) and ANAPC5 knockout (KO) 293T cells, using Anti-Apc5 Antibody (A15547) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Apc5 Antibody (A15547) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-Apc5 Antibody (A15547) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 5: Western Blot - Anti-Apc5 Antibody (A15547)
Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg of Anti-Apc5 Antibody (A15547). This Western blot was performed on the immunoprecipitate using Anti-Apc5 Antibody (A15547) at a dilution of 1:1000.
