This antibody recognises Apaf1 (Apoptosis protease activating factor 1), the mammalian homologue of the CED-4 cell death protein in C. Elegans. Apaf1 binds to cytochrome C and caspase-9 leading to caspase-9 activation, resulting in the beginning of the proteolytic cascade characteristic of apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. recognises a ~130 kDa band in western blots.
Applicazioni
WB, IHC-P
Diluizioni consigliate
WB: 1:1,000 - 1:2,000
Reattività
Human, Mouse
Immunogeno
Synthetic peptide corresponding to amino acids 12-28 of human Apaf1.
Sequenza
HREALEKDIKTSYIMDH
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide.
Conservazione
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Note generali
Rabbit anti Apaf1 antibody recognizesApaf1 (Apoptosis protease activating factor 1), the mammalian homologue of the CED-4 cell death protein in C. Elegans. Apaf1 binds to cytochrome C and caspase-9 leading to caspase-9 activation, resulting in the beginning of the proteolytic cascade characteristic of apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Rabbit anti Apaf1 antibody (AHP487) recognizes a ~130 kDa band in western blots.
