Figure 1: Western Blot - Anti-Angiopoietin 1 Antibody [ARC0248] (A305757)
Western blot analysis of extracts of various cell lines, using Anti-Angiopoietin 1 Antibody [ARC0248] (A305757) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Angiopoietin 1 Antibody [ARC0248] (A305757) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Prodotti alternativi a Anti-Angiopoietin 1 Anticorpo [ARC0248] (A305757)