Figure 1: Western Blot - Anti-Angiopoietin 1 Antibody (A84861)
Angiopoietin 1 expression in K56 cell lysate (A) + peptide (B) analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Angiopoietin 1 Antibody (A84861) at 0.1µg/ml and detected by chemiluminescence.
Angiopoietin 1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Angiopoietin 1 Antibody (A84861) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Angiopoietin 1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Angiopoietin 1 Antibody (A84861) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Angiopoietin 1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Angiopoietin 1 Antibody (A84861) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
Figure 5: Western Blot - Anti-Angiopoietin 1 Antibody (A84861)
Angiopoietin 1 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress FLAG tagged Angiopoietin 1 an lysed in RIPA buffer. Primary incubation was performed for 1 hour on 10µg protein per lane with Anti-Angiopoietin 1 Antibody (A84861) at 1µg/ml (Lane A) or Anti-FLAG at 1:1000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-Angiopoietin 1 Antibody (A84861) at 1mg/ml (Lane B).
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