Mouse monoclonal [AFP-01] antibody to alpha Fetoprotein (Biotin).
Specificità
This antibody reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Applicazioni
WB, ICC, ELISA, IP
Diluizioni consigliate
ELISA: This antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha fetoprotein in combination with A85534, WB: 1-2 µg/ml; Non-reducing conditions, IP: Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
Reattività
Human
Immunogeno
Purified alpha Fetoprotein.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
AFP-01
Isotipo
IgG1
Coniugare
Biotin
Purificazione
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentrazione
1 mg/ml
MW previsto
70 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.