Anti-alpha B Crystallin Anticorpo [3A10.C9] (A304738)

Western blot analysis of rat brain, heart, kidney, liver, pancreas, skeletal muscle, spleen, testes, thymus cell lysates showing detection of Alpha B Crystallin protein using Anti-alpha B Crystallin Antibody [3A10.C9] (A304738) at 1:50 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-alpha B Crystallin Antibody [3A10.C9] (A304738), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha B Crystallin Antibody. (D) Composite.