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Anti-Alkaline Phosphatase, Tissue Non-Specific Anticorpo (A91218)

Western Blot - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.com Immunohistochemistry - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.com
$335
Product Datasheet

Anticorpo policlonale di coniglio contro Alkaline Phosphatase, Tissue Non-Specific per WB e IHC.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
5-8 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody
Descrizione
Rabbit polyclonal antibody to Alkaline Phosphatase, Tissue Non-Specific.
Applicazioni
WB, IHC
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 230-440 of human Alkaline Phosphatase (ALPL) (NP_000469.3).
Sequenza
NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNN
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
80 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
AKP2, Alkaline phosphatase liver/bone/kidney, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphomonoesterase, Alpl, AP TNAP, AP-TNAP, APTNAP, BAP, FLJ40094, FLJ93059, Glycerophosphatase, HOPS, Liver/bone/kidney type alkaline phosphatase, MGC161443, MGC167935, PHOA, PPBT_HUMAN, Tissue non specific alkaline phosphatase, Tissue nonspecific ALP, TNAP, TNSALP
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218)
Western blot analysis of extracts of HeLa cells, using Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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