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Anti-ALG5 Anticorpo (A307584)

Nome
Anti-ALG5 Antibody
Descrizione
Rabbit polyclonal antibody to ALG5.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 34-190 of human ALG5 (NP_037470.1).
Sequenza
MPALHRHEEEKFFLNAKGQKETLPSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSKDQTSKVAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDVEKLEKGLNDLQPWPNQM
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
37 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Alg5, S. cerevisiae, homolog of, ALG5_HUMAN, Asparagine linked glycosylation 5, Asparagine-linked glycosylation protein 5 homolog, Dolichyl phosphate beta glucosyltransferase, Dolichyl-phosphate beta-glucosyltransferase, DolP glucosyltransferase, DolP-glucosyltransferase
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-ALG5 Antibody (A307584) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-ALG5 Antibody (A307584)
Western blot analysis of extracts of various cell lines, using Anti-ALG5 Antibody (A307584) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-ALG5 Antibody (A307584) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-ALG5 Antibody (A307584)
Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Anti-ALG5 Antibody (A307584) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-ALG5 Antibody (A307584) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-ALG5 Antibody (A307584)
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-ALG5 Antibody (A307584) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-ALG5 Antibody (A307584) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-ALG5 Antibody (A307584)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-ALG5 Antibody (A307584) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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