Anti-AKT1 (phospho Ser473) Anticorpo (A16468)

Western blot analysis of extracts of Jurka cells, using Anti-AKT1 (phospho Ser473) Antibody (A16468) at 1:1,000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37°C. Jurkat cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts of C6 cells, using Anti-AKT1 (phospho Ser473) Antibody (A16468) at 1:1,000 dilution. C6 cells were treated by Serum-starvation overnight at 37°C. C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 10s.