Figure 1: Western Blot - Anti-AKT1 Antibody (A285973)
AKT1 expression in MCF7 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-AKT1 Antibody (A285973) at 0.3µg/ml and detected by chemiluminescence.
AKT1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-AKT1 Antibody (A285973) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/microtubule staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Prodotti alternativi a Anti-AKT1 Anticorpo (A285973)