The antibody detects endogenous level of AKT1 / AKT2 / AKT3 only when phosphorylated at tyrosine 315 / 316 / 312.
Applicazioni
WB, IHC, IF
Diluizioni consigliate
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reattività
Human, Mouse, Rat
Immunogeno
Peptide sequence around phosphorylation site of tyrosine 315 / 316 / 312 (P-E-Y(p)-L-A) derived from Human AKT1 / AKT2 / AKT3.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentrazione
1.0mg / ml
MW previsto
60kd
Formulazione
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Conservazione
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Sinonimi
AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) Antibody #11501 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 3T3 cells, treated with EGF or calf intestinal phosphatase (CIP), using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) Antibody #11501.