Recombinant rabbit monoclonal [RM251] antibody to AKT (phospho Ser473).
Specificità
This antibody recognizes AKT only when phosphorylated at Ser473.
Applicazioni
WB, IHC
Diluizioni consigliate
IHC: 1:200-1:500, WB: 1:1,000-1:2,000
Reattività
Human
Reattività incrociata
This antibody does not cross-react with AKT without phosphorylation at Ser473. This antibody may react with samples from bovine, mouse, and rat, as predicted by immunogen homology.
Immunogeno
Synthetic peptide corresponding to human AKT (phospho Ser473).
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
RM251
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Protein A chromatography.
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Figure 1: Western Blot - Anti-AKT (phospho Ser473) Antibody [RM251] (A121248)
Western blot of lysates from 293 cells, either grown in medium with serum, serum starved, or insulin treated, using Anti-AKT (phospho Ser473) Antibody [RM251] at a 1:1,000 dilution or Anti-AKT1 Antibody [RM252] at a 1:1,000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-AKT (phospho Ser473) Antibody [RM251] at 1:400 dilution.
Prodotti alternativi a Anti-AKT (phospho Ser473) Anticorpo [RM251] (A121248)
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