The antibody detects endogenous level of total Akt protein.
Applicazioni
WB, IHC
Diluizioni consigliate
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reattività
Human, Mouse, Rat
Immunogeno
Peptide sequence around aa.306~310 (M-K-T-F-C) derived from Human AKT1.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentrazione
1.0mg / ml
MW previsto
60kd
Formulazione
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Conservazione
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Ab-308) Antibody #21055 (left) or the same antibody preincubated with blocking peptide (right).
Prodotti alternativi a Anti-Akt (Ab-308) Anticorpo (A36520)