Anti-Ago1 Antibody (A82458)

Name: Anti-Ago1 Antibody
Brand: Antibodies.com
SKU: A82458
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to Ago1 for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-Ago1 Antibody

Description

Goat polyclonal antibody to Ago1.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:16,000, WB: 0.3-1µg/ml, IHC: 2.5µg/ml

Reactivity

Human

Cross Reactivity

This antibody is not expected to cross react with EIF2C2, EIF2C3 and EIF2C4.

Immunogen

Synthetic peptide corresponding to the internal region of human Ago1.

Sequence

C-KNASYNLDPYIQEF

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

110 kDa and 35 kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Argonaute RISC catalytic component 1, Argonaute1, eIF-2C 1, eIF2C 1, EIF2C1, Eukaryotic translation initiation factor 2C 1, hAgo1, Protein argonaute-1, Putative RNA-binding protein Q99

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Ago1 Antibody (A82458)

Ago1 expression in HEK293 cell lysate transfected (A) and untransfected (B) analyzed by western blot. Cells were transfected to overexpress Ago1. Staining was performed with Anti-Ago1 Antibody (A82458).

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Immunohistochemistry - Anti-Ago1 Antibody (A82458)

Ago1 expression in Human Placenta analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Ago1 Antibody (A82458) at 2.5µg/ml and revealed with alkaline phosphatase (AP).

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Immunohistochemistry - Anti-Ago1 Antibody (A82458)

Ago1 expression in Human Small Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Ago1 Antibody (A82458) at 2.5µg/ml and revealed with alkaline phosphatase (AP).

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