Anti-ADAM12 Anticorpo (A83260)

Name: Anti-ADAM12 Antibody
Brand: Antibodies.com
SKU: A83260
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab28747). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro ADAM12 per ELISA, Flow Cytometry, IF e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-ADAM12 Antibody

Descrizione

Goat polyclonal antibody to ADAM12.

Applicazioni

ELISA, Flow Cytometry, IF, IHC

Diluizioni consigliate

ELISA: 1:64,000, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Reattività incrociata

This antibody does not cross react with other ADAMS.

Immunogeno

Synthetic peptide corresponding to the N terminal region of human ADAM12.

Sequenza

AARPLPVSPARALC

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

A disintegrin and metalloproteinase domain 12, ADA12_HUMAN, ADAM 12, ADAM metallopeptidase domain 12, CAR10, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, Meltrin alpha, Meltrin-alpha, Metalloprotease disintegrin 12 transmembrane, MLTN, MLTNA, OTTHUMP00000046766

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Immunofluorescence - Anti-ADAM12 Antibody (A83260)

ADAM12 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ADAM12 Antibody (A83260) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic/plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

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Figure 2: Flow Cytometry - Anti-ADAM12 Antibody (A83260)

ADAM12 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ADAM12 Antibody (A83260) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 0.4µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 3: Immunohistochemistry - Anti-ADAM12 Antibody (A83260)

ADAM12 expression in Human Prostate analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ADAM12 Antibody (A83260) at 3.8µg/ml and revealed with alkaline phosphatase (AP).

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Figure 4: Immunohistochemistry - Anti-ADAM12 Antibody (A83260)

ADAM12 expression in Human Placenta analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ADAM12 Antibody (A83260) at 3.75µg/ml and revealed with alkaline phosphatase (AP).