Anti-ABCE1 Anticorpo (A326209)

Name: Anti-ABCE1 Antibody
Brand: Antibodies.com
SKU: A326209
Price: 448.0 USD
Availability: InStock

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$448

Anticorpo policlonale di capra contro ABCE1 per ELISA, WB, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-ABCE1 Antibody

Descrizione

Goat polyclonal antibody to ABCE1.

Applicazioni

ELISA, WB, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:32,000, WB: 1-2µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the N terminal region of human ABCE1.

Sequenza

NHDKCKPKKCRQEC

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

70 kDa

MW previsto

67.3kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

2' 5' oligoadenylate binding protein, 2''-5''-oligoadenylate-binding protein, 2'5' oligoadenylate binding protein, ABC 38, ABC38, ABCE 1, ABCE1_HUMAN, ATP binding cassette sub family E (OABP) member 1, ATP binding cassette sub family E member 1, ATP-binding cassette sub-family E member 1, HuHP 68, HuHP68, OABP, Ribonuclease 4 inhibitor, Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) inhibitor, Ribonuclease L (2'5' oligoisoadenylate synthetase dependent) inhibitor, Ribonuclease L inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS 4I, RNS4I

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-ABCE1 Antibody (A326209)

ABCE1 expression in Human Tonsil lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ABCE1 Antibody (A326209) at 2µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-ABCE1 Antibody (A326209)

ABCE1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ABCE1 Antibody (A326209) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-ABCE1 Antibody (A326209)

ABCE1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ABCE1 Antibody (A326209) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-ABCE1 Antibody (A326209)

ABCE1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ABCE1 Antibody (A326209) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.