Anti-5HT2A Receptor Anticorpo (A82980)

Name: Anti-5HT2A Receptor Antibody
Brand: Antibodies.com
SKU: A82980
Price: 480.0 USD
Availability: InStock

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$480

Anticorpo policlonale di capra contro 5HT2A Receptor per ELISA, IHC, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-5HT2A Receptor Antibody

Descrizione

Goat polyclonal antibody to 5HT2A Receptor.

Applicazioni

ELISA, IHC, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:128,000, IHC: 2µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region, amino acids 296-309, of human 5HT2A Receptor.

Sequenza

C-QRSIHREPGSYTGR

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

5 HT 2, 5 HT 2A, 5 HT2 receptor, 5 HT2A, 5 hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled, 5-hydroxytryptamine 2A receptor, 5-hydroxytryptamine receptor 2A, 5HT2A_HUMAN, HTR 2, HTR 2A, HTR2, HTR2, formerly, HTR2A, serotonin 5-HT-2 receptor, formerly, serotonin 5-HT-2A receptor, Serotonin receptor 2A

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Immunohistochemistry - Anti-5HT2A Receptor Antibody (A82980)

5HT2A Receptor expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-5HT2A Receptor Antibody (A82980) at 2µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 2: Immunohistochemistry - Anti-5HT2A Receptor Antibody (A82980)

Negative control for 5HT2A Receptor expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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Figure 3: Immunofluorescence - Anti-5HT2A Receptor Antibody (A82980)

5HT2A Receptor expression in A549 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-5HT2A Receptor Antibody (A82980) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-5HT2A Receptor Antibody (A82980)

5HT2A Receptor expression in A549 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-5HT2A Receptor Antibody (A82980) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.